Dynamics of pH-induced spectral changes in bacteriorhodopsin
نویسندگان
چکیده
منابع مشابه
Electric-field induced pK-changes in bacteriorhodopsin.
Bacteriorhodopsin, the only protein of the purple membranes of halobacteria [ 11, acts as a light-driven proton pump, translocating protons from the inner to the outer side of the plasma membrane [2]. The lightinduced asymmetric proton distribution contributes to the membrane potential by up to lo-40 mV [3]. Assuming a membrane thickness of 50 A, this potential difference corresponds to an elec...
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Bacteriorhodopsin (bR) trimers naturally form two-dimensional hexagonal crystals in purple membrane (PM), which make it very stable. However, the dnaturation of bR was found to occur during a very narrow pH range when the pH was increased above 12.0, as indicated by inactivation of the photochemical cycle observed by flash photolysis kinetic spectra. Here, atomic force microscopy was used to st...
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Electric impulses of high field intensity (2 x 105 to 3 × 106 Vm1, 1 to 20 #s duration) cause transient changes in the optical absorbance of suspended purple membranes ofHalobacterium halobium. The electric dichroism at 1 mM NaCl, pH ~ 6,and at 293K is dependent on field strength, pulse duration and wavelength of the monitoring, plane-polarized light in the range 400 to 650 nm. The optically de...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1979
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(79)85241-8